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Общее количество найденных документов : 27
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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pershina A. G., Demin A. M., Perekucha N. A., Brikunova O. Y., Efimova L. V., Nevskaya K. V., Vakhrushev A. V., Zgoda V. G., Uimin M. A., Minin A. S., Malkeyeva D., Kiseleva E., Zima A. P., Krasnov V. P., Ogorodova L. M.
Заглавие : Peptide ligands on the PEGylated nanoparticle surface and human serum composition are key factors for the interaction between immune cells and nanoparticles
Место публикации : Colloids and Surfaces B: Biointerfaces. - 2023. - Vol. 221. - С. 112981
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Аннотация: The architecture of a nanoparticles' surface formed due to a modification with a ligand and protein corona formation in biofluids is critical for interactions with cells in vivo. Here we studied interactions of immune cells with magnetic nanoparticles (MNPs) covalently modified with polyethylene glycol (PEG) and their counterparts conjugated with peptides: a pH (low) insertion peptide (pHLIP) and cycloRGD as a targeting ligand in human serum. The conjugation of MNPs-PEG with pHLIP, but not with cycloRGD, enhanced the association of these particles with mononuclear phagocytic cells in vitro and in vivo. We did not find a clear difference in protein corona composition between the pHLIP-modified and parental PEGylated nanoparticles. Analysis of the effect of autologous human serum on MNP uptake by monocytes showed that the efficiency of endocytosis varies among healthy donors and depends on intrinsic properties of serum. Nevertheless, using classic blood, coagulation, biochemical tests, and anti-PEG IgG serum level, we failed to identify the cause of the observed interdonor variation. These individual differences should be taken into consideration during testing of nanotherapeutics.
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khonina T. G., Nikitina E. Y., Tishin D. S., Demin A. M., Krasnov V. P., Chupakhin O. N., Charushin V. N., Germov A. Y., Goloborodsky B. Y., Mikhalev K. N., Bogdanova E. A.
Заглавие : Individual iron(III) glycerolate: synthesis and characterisation
Место публикации : RSC Advances. - 2022. - Vol. 12, № 7. - С. 4042-4046
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Аннотация: Iron(II) and iron(III) salts of strong acids form iron glycerolates on heating at 180 °C with glycerol in the presence of an equivalent amount of alkali. Individual iron(III) glycerolate was obtained for the first time. When Fe3O4 magnetic nanoparticles were heated with glycerol, an iron(III) glycerolate shell was formed on their surface.
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3.

Вид документа : Статья из журнала
Шифр издания : Г/S 98
Автор(ы) : Bakhteeva I. A., Medvedeva I. V., Byzov I. V., Konev A. S., Zhakov S. V., Uimin M. A., Demin A. M., Murzakaev A. M., Medvedeva O. M.
Заглавие : Synthesis of Fe@C nanoparticles containing sulfo groups on their surfaces and study of their aggregation behavior in aqueous media
Место публикации : Russian chemical bulletin. - 2021. - Vol. 70, № 4. - С. 722-731
ББК : Г
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): magnetic nanoparticles--iron-carbon--benzenesulfonic acid--ir spectroscopy
Аннотация: Magnetic iron nanoparticles (MNPs) encapsulated in a carbon shells and containing sulfo groups on the surface (Fe@C-SO3H) were synthesized. The aggregative stability of aqueous suspensions of the Fe@C-SO3H nanoparticles was studied in solutions of bovine serum albumin and calcium chloride and in phosphate-buffered saline, which simulate blood plasma. It is shown that the Fe@C-SO3H particles practically do not aggregate in the phosphate buffer saline for a long time (several days). On the contrary, MNPs encapsulated in a protein shells using ultrasonic treatment form aggregates up to 110–120 nm in size under these conditions. Suspensions of the Fe@C-SO3H particles are stable in aqueous solutions of calcium chloride in the range of the solid phase concentration between 0.05 and 0.10 g L−1. The results obtained indicate the possibility of using the functionalized Fe@C-SO3H nanoparticles in vitro experiments in biological media.
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4.

Вид документа : Статья из журнала
Шифр издания : Г/M 78
Автор(ы) : Demin A. M., Vakhrushev A. V., Mekhaev A. V., Krasnov V. P., Uimin M. A.
Заглавие : Modification of Fe3O4 magnetic nanoparticles with a GRGD peptide
Место публикации : Russian chemical bulletin. - 2021. - Vol. 70, № 3. - С. 449-456
ББК : Г
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): magnetic nanoparticles--rgd peptides--peptide synthesis
Аннотация: Derivatives of RGD peptides are promising vector molecules for targeting biomolecules and nanoparticles into tumor tissues and are used for the design of diagnostic agents. The immobilization of the glutaryl-containing derivative of Gly-L-Arg(Pbf)-Gly-L-Asp(OMe)2 (GRGD) tetrapeptide on Fe3O4 magnetic nanoparticles (MNPs) was studied. The results of thermogravimetric and elemental analysis, as well as IR spectroscopy, were used to estimate the amount of organic components at each stage of modification of MNPs.
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5.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Demin A. M., Maksimovskikh A. I., Mekhaev A. V., Krasnov V. P., Kuznetsov D. K., Shur V. Y., Minin A. S., Uimin M. A., Pershina A. G.
Заглавие : Silica coating of Fe3O4 magnetic nanoparticles with pmida assistance to increase the surface area and enhance peptide immobilization efficiency
Место публикации : Ceramics international. - 2021. - № б/н. - С. 1-6
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
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6.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Demin A. M., Kandarakov O. F., Minin A. S., Kuznetsov D. K., Uimin M. A., Shur V. Ya., Belyavsky A. V., Krasnov V. P.
Заглавие : Modification of chemically and physically obtained Fe3O4 magnetic nanoparticles with L-Lys for cell labeling
Место публикации : Russian chemical bulletin. - 2021. - Vol. 70, № 6. - С. 1199-1208
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Аннотация: Peculiar features of the modification of Fe3O4 magnetic nanoparticles (MNPs) obtained by co-precipitation from solutions of FeII and FeIII salts (10-nm MNPs) and by the gas-condensation method (30-nm MNPs) with 3-aminopropylsilane and then with L-lysine were studied. The chemically obtained MNPs possess more pronounced hydrophilic properties and therefore readily form stable aqueous colloidal solutions and can be loaded with a larger amount of L-Lys. However, samples based on the physically obtained MNPs were characterized by more efficient internalization and longer retention times by the NIH 3T3 cells. The results obtained can be used in the design of novel nanomaterials for magnetic cell labeling.
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7.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pershina A. G., Brikunova O. Y., Vtorushin S. V., Gereng E. A., Ogorodova L. M., Timin A. S., Demin A. M., Krasnov V. P., Abakumov M. A., Vaneev A. N.
Заглавие : Variation in tumor pH affects pH-triggered delivery of peptide-modified magnetic nanoparticles
Место публикации : Nanomedicine: nanotechnology, biology and medicine. - 2021. - Vol. 32. - С. 102317
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): ph profile--endocytosis--iron oxide magnetic nanoparticles
Аннотация: Acidification of the extracellular matrix, an intrinsic characteristic of many solid tumors, is widely exploited for physiologically triggered delivery of contrast agents, drugs, and nanoparticles to tumor. However, pH of tumor microenvironment shows intra- and inter-tumor variation. Herein, we investigate the impact of this variation on pH-triggered delivery of magnetic nanoparticles (MNPs) modified with pH-(low)-insertion peptide (pHLIP). Fluorescent flow cytometry, laser confocal scanning microscopy and transmission electron microscopy data proved that pHLIP-conjugated MNPs interacted with 4T1 cells in two-dimensional culture and in spheroids more effectively at pH 6.4 than at pH 7.2, and entered the cell via clathrin-independent endocytosis. The accumulation efficiency of pHLIP-conjugated MNPs in 4T1 tumors after their intravenous injection, monitored in vivo by magnetic resonance imaging, showed variation. Analysis of the tumor pH profiles recorded with implementation of original nanoprobe pH sensor, revealed obvious correlation between pH measured in the tumor with the amount of accumulated MNPs.
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8.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Demin A. M., Vakhrushev A. V., Valova M. S., Minin A. S., Kuznetsov D. K., Uimin M. A., Shur V. Ya., Krasnov V. P., Charushin V. N.
Заглавие : Design of SiO2/aminopropylsilane-modifi ed magnetic Fe3O4 nanoparticles for doxorubicin immobilization
Место публикации : Russian chemical bulletin. - 2021. - Vol. 70, № 5. - С. 987-994
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): magnetic nanoparticles --n-phosphonomethyliminodiacetic acid--doxorubicin--sio2 coating--sorption
Аннотация: Surface modifi cation of magnetic nanoparticles (MNPs) with tetramethylorthosilicate and 3-aminopropyltrimethoxysilane was studied. The use of N-phosphonomethyliminodiacetic acid for the stabilization of the initial MNPs can signifi cantly increase the coating thickness and specifi c surface area of SiO2/APS-modifi ed (APS is 3-aminopropylsilane) MNPs. The possibility of sorption of antitumor drug doxorubicin (DOX) on the synthesized nanoparticles was studied. The degree of sorption of DOX increases with an increase in the specifi c surface area of the particles.
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9.

Вид документа : Статья из журнала
Шифр издания : 24/I-55
Автор(ы) : Demin A. M., Mekhaev A. V., Krasnov V. P., Esin A. A., Kuznetsov D. K., Zelenovskiy P. S., Shur Y. Y.
Заглавие : Immobilization of pmida on Fe3O4 magnetic nanoparticles surface: mechanism of bonding
Место публикации : Applied surface science. - 2018. - V 440. - С. 1196-1203
ББК : 24
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): ftir--magnetic nanoparticles--mechanism of immobilization--pmida--tga--xps
Аннотация: The mechanism of N-phosphonomethyl iminodiacetic acid (PMIDA) binding with the Fe3O4 magnetic nanoparticle (MNPs) surface by Fourier transformed infrared spectroscopy, X-ray photoelectron spectroscopy and thermogravimetry was comprehensive studied. To study of microstructure, size and core structure of synthesized nanoparticles the scanning electron microscopy, X-ray diffraction analysis and Raman spectroscopy were carried out. A new scheme for the tridentate bonding of the phosphonomethyl derivative with surface Fe atoms involving unequal P–O–Fe bonds was proposed. The mechanism of thermal decomposition of PMIDA molecules on the MNP surface was studied using a thermogravimetric analyzer combined with infrared spectrometer. It was shown for the first time that during the thermal treatment of phosphonomethyl-modified MNPs, PMIDA molecules are not desorbed from the surface of MNPs but gradually decompose. We believe that obtained in this work data will be useful for a deeper understanding of the mechanisms of phosphonic acid derivatives interaction with MNPs, as well as in the design of new biomedical materials, in which the conjugation of biomolecules with carboxyl groups of PMIDA-modified MNPs is assumed.
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10.

Вид документа : Статья из журнала
Шифр издания : 54/H 53
Автор(ы) : Pershina A. G., Saltykova I. V., Ivanov V. V., Demin A. M., Buzueva I. I., Ganebnuikh I. N., Krasnov V. P., Ogorodova L. M.
Заглавие : Hemozoin “knobs” in Opisthorchis felineus infected liver
Место публикации : Parasites & Vectors. - 2015. - Vol. 8. - С. 459
ББК : 54
Предметные рубрики: ХИМИЧЕСКИЕ НАУКИ
Ключевые слова (''Своб.индексиров.''): opisthorchis felineus --knobs--hemozoin
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